Pall Filtration Sensor Chip Series S Sa Certified, 3 Items GEHEBR-1005-31

Cytiva - Biocore Sensor Chip, Series S, SA
For interaction analysis using biotinylated molecules

This high-affinity streptavidin (SA) sensor chip immobilizes biotinylated molecules for surface plasmon resonance (SPR) interaction analysis on Biacore systems.

• Versatile: immobilizes biotinylated peptides, proteins, nucleic acids, or carbohydrates
  
  • Ready-to-use: streptavidin is pre-immobilized, ready for fast, high-affinity ligand capture
  • Robust: streptavidin-biotin is one of the strongest non-covalent interactions in nature
  • Non-covalent: a convenient alternative for ligands refractory to covalent immobilization
  • Standardized ligand orientation: controlled biotinylation enables oriented capture

Streptavidin-biotin capture
Sensor Chip SA provides a surface of streptavidin covalently attached to a carboxymethyldextran matrix. Dissociation of biotinylated ligands from the surface of the chip is generally negligible during the course of a Biacore analysis because the affinity of streptavidin for biotin is extremely high, with an equilibrium dissociation constant of about 10-15 M. High binding capacity, reproducibility, and chemical resistance give excellent performance over a broad range of applications. The surface of Sensor Chip SA is resistant to 1-minute pulses of many agents commonly used in SPR sensor chip washing and regeneration protocols.

Not recommended for use with Biacore 4000, Biacore A100 or Biacore S51