
Pall Filtration Pgex-6P-1 Vector, 25 µg GEHE28-9546-48
Pall Filtration -- Item TECPIM000568433
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The pGEX vectors have an expanded multiple cloning site (MCS) that contains six restriction sites. The expanded MCS facilitates the unidirectional cloning of cDNA inserts obtained from libraries constructed using many available lambda vectors. pGEX-6P-1, pGEX-6P-2, and pGEX-6P-3 each encode the recognition sequence for site-specific cleavage by PreScission Protease between the GST domain and the multiple cloning site. pGEX-4T-1, pGEX-4T-2, and pGEX-4T-3 are derived from pGEX-2T and contain a Thrombin recognition site. pGEX-5X-1, pGEX-5X-2, and pGEX5X-3 are derivatives of pGEX-3X and possess a Factor Xa recognition site.
- A tac promoter for chemically inducible, high-level expression of GST-tagged recombinant proteins An internal lacIq gene for use in any E. coli host Very mild elution conditions for release of fusion proteins from the affinity matrix, thus minimising effects on antigenicity and functional activity PreScission Protease, Thrombin, or Factor Xa recognition sites for cleaving the desired protein from the fusion product
Cleavage of pGEX-6P GST fusion proteins occurs between the Gln and Gly residues of the recognition sequence Leu-Glu-Val-Leu-Phe-Gln-Gly-Pro. Low temperature (5 °C) digestion minimises the degradation of the protein of interest. Because PreScission Protease has been engineered with a GST tag, it can also be removed from the cleavage mixture simultaneously with the GST portion of the fusion protein. The pGEX-6P Expression Vectors permit convenient site-specific cleavage and simultaneous purification on Glutathione Sepharose. The pGEX-6P series provides all three translational reading frames linked between the GST coding region and the multiple cloning site.
Collectively, the pGEX vectors provide all three translational reading frames beginning with the EcoRI restriction site. pGEX-1µT, pGEX-6P-1, pGEX-4T-1, and pGEX-5X-1 can directly accept and express cDNA inserts isolated from µgt11 libraries.
Pack contains pGEX vector only (E. coli BL21 available separately).
Product Details
Model | |
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Product Type | Consumables |
Part Number | GEHE28-9546-48 |
Brand | Pall Filtration |
Gross Dimensions (WxDxH cm) | |
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Gross Weight (kg) | |
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More options are available. Please contact us for more information.
More options are available. Please contact us for more information.