Pall Filtration Column Hitrap Imac FF 5 X 5ml, 5 Items GEHE17-0921-04

HiTrap™ IMAC FF for Immobilized Metal ion Affinity Chromatography (IMAC). The columns are prepacked with IMAC Sepharose™ 6 Fast flow for subsequent high-resolution purification of histidine-tagged proteins when Ni²? is not the best choice of metal ion.

• Stable over pH 3 ? 12 and tolerates all commonly used aqueous buffers Optimal bead size for scaling up High binding capacity

IMAC Sepharose™ FF is supplied free of metal ions. It is charged by the user with the transition metal ion of choice (e.g. Cu²⁺, Zn²⁺, Ni²⁺, or Co²⁺); these metal ions will bind to the covalently immobilized chelating ligand on the Sepharose™. IMAC Sepharose™ 6 Fast Flow is highly stable and compatible with a wide range of common additives.