Pall Filtration Column FF Crude Histrap 5X5 ml, 5 Items GEHE17-5286-01

HisTrap™ FF crude columns offer the convenience of simple, one-step purification of histidine-tagged proteins directly from homogenized, unclarified cell lysate by immobilized metal ion affinity chromatography (IMAC). The columns are prepacked with precharged Ni Sepharose™ 6 Fast Flow. Ni Sepharose™ 6 Fast Flow has low nickel ion (Ni²⁺) leakage and is compatible with a wide range of additives used in protein purification.

• High binding capacity and low nickel ion leakage Ensures reliable capture of histidine-tagged proteins in repeated IMAC purifications Compatible with a wide range of reducing agents, detergents, denaturants and other additives Simple manual operation with a syringe, pump or chromatography system such as ÄKTA design or FPLC system