Cytiva NI Sepharose Excel, 500 ml GEHE17-3712-03

Ni Sepharose™ excel is an IMAC medium pre-charged with nickel ions very strongly bound to a chelating ligand. Samples that usually cause stripping of metal ions can therefore be loaded to the medium. Ni Sepharose™ excel is designed primarily for capture and purification of histidine-tagged proteins secreted into cell culture supernatants from eukaryotic cells such as insect cells or CHO cells. Increased target protein yield Minimised degradation through reduced and simplified sample handlingNi Sepharose™ excel enables direct loading of samples without having to perform extensive and time consuming pre-treatment procedures. The flow properties of the medium make it excellent for purifications in a wide range of scales and allow loading of large sample volumes, enabling purification of low concentrations of target proteins at large volumes.